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Design and optimization of a cell-free atrazine biosensor

Description:

Recent advances in cell-free synthetic biology have spurred the development of in vitro molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However, cell-free sensors for detecting manmade organic water contaminants such as pesticides are sparse, partially because few characterized natural biological sensors can directly detect such pollutants. Here,...

Keyword:

biosensor, TX-TL, atrazine, cell-free, cyanuric acid, metabolism, synthetic biology, and transcription factor

Creator:

Umut Akova, Khalid K. Alam, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman

Owner:

Adam Silverman

Date Uploaded:

09/23/2019

Date Modified:

02/19/2020
Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry

Description:

Recent advances in cell-free gene expression (CFE) systems have enabled their use for a host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and biosensors. Despite the proliferation of cell-free protein synthesis platforms, the large number of currently existing protocols for making CFE extracts muddles the collective...

Keyword:

cell-free synthetic biology, TX-TL, CFE, cell extract, genetic circuitry, in vitro protein synthesis, CFPS, and endogenous transcription

Subject:

Synthetic biology and Genetic regulation

Creator:

Nancy Kelley-Loughnane, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman

Owner:

Julius Beau Lucks

Publisher:

American Chemical Society

Language:

English

Date Uploaded:

12/14/2018

Date Modified:

01/07/2020

Date Created:

12/14/18

Resource Type:

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Design and optimization of a cell-free atrazine biosensor

Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry

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