In the field of clinical diagnostics, biosensors are used to identify genetic
disorders by hybridizing a surface-bound DNA sequence with the
patient’s DNA. To further improve biosensor design, two nonlinear
optical techniques, sum frequency generation (SFG) and second
harmonic generation (SHG), were used to probe the orientation,
chirality, and surface...
Second harmonic generation (SHG),
a nonlinear optical tool, was used to
characterize DNA bound through a
chemical linker to the flat surface of a
hemispherical lens. Contact angle
measurements were used to guarantee
that the chemical linker did not hydrolyze
under experimental conditions. SHG
experiments followed the effects of salt...