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Generation and Elucidation of Complex Bacterial Phenotypes Using High-Throughput Genomic Techniques

Description:

Using a novel DNA-microarray hybridization protocol, the diffusion distance during static microarray hybridization was estimated for Cy3- and Cy5-labeled cDNA probes as 3.8 and 2.6 mm, respectively, despite having almost identical molecular masses. Continuous mixing during microarray hybridization resulted in a 15-20% increase in signal intensity over arrays hybridized statically....

Keyword:

butanol, biofuel, DNA microarray, Clostridium acetobutylicum, and Genomic library

Subject:

Chemical and Biological Engineering

Creator:

Jacob Reece Borden

Owner:

Scholarly Digital Publishing

Date Uploaded:

08/27/2018

Date Modified:

08/27/2018

Date Created:

2007-10-19

Resource Type:

Dissertation
METABOLIC ENGINEERING OF CLOSTRIDIUM ACETOBUTYLICUM FOR ENHANCED BUTANOL PRODUCTION AND SELECTIVITY

Description:

To increase the butanol titers and selectivity in Clostridium acetobutylicum we replaced the promoter of the alcohol/aldehyde dehydrogenase (aad) gene with the phosphotranbutyrylase (ptb) promoter and combined this with CoAT downregulation to minimize acetone production. This led to early production of high alcohol (butanol plus ethanol) titers and overall solvent...

Keyword:

metabolic engineering and Clostridium acetobutylicum

Subject:

Chemical and Biological Engineering

Creator:

William Sillers

Owner:

Scholarly Digital Publishing

Date Uploaded:

10/01/2018

Date Modified:

10/01/2018

Date Created:

2008-12-04

Resource Type:

Dissertation

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Generation and Elucidation of Complex Bacterial Phenotypes Using High-Throughput Genomic Techniques

METABOLIC ENGINEERING OF CLOSTRIDIUM ACETOBUTYLICUM FOR ENHANCED BUTANOL PRODUCTION AND SELECTIVITY

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