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Using SAMDI-MS and Peptide Arrays to Study Protein Tyrosine Phosphatases and Discover New Cellular Regulatory Mechanisms

Description:

The opposing activities of phosphatases and kinases determine the phosphorylation status of proteins, yet kinases have received disproportionate attention in studies of cellular processes, with the roles of phosphatases remaining less understood. This dissertation describes the use of self-assembled monolayer laser desorption/ionization mass spectrometry (SAMDI-MS) together with peptide arrays to...

Keyword:

SAMDI-MS, Phosphatases, High-Throughput Experiments, Enzyme Specificity, β-Catenin, and Peptide Arrays

Subject:

Chemistry, Biochemistry, and Cellular biology

Creator:

Huang, Che-Fan

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

06/28/2021

Date Created:

2021-01-01

Resource Type:

Dissertation

Alternate Identifier:

etdadmin_upload_819016 and http://dissertations.umi.com/northwestern:15570
Applications of SAMDI Mass Spectrometry for High-Throughput Reaction Monitoring on Peptide Arrays

Description:

This work combines the use of high-throughput mass spectrometry with peptide arrays for to monitor reactions on peptides. The Mrksich lab introduced a high-throughput, label-free, biochemical assay that relies on self-assembled monolayers on gold and matrix-assisted laser desorption/ionization mass spectrometry, termed SAMDI-MS. This dissertation introduces new applications of SAMDI-MS and...

Keyword:

High-throughput, SAMDI, Phosphatase, Mass spectrometry, and Peptide Arrays

Subject:

Chemistry and Biochemistry

Creator:

Szymczak, Lindsey Catherine

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

02/12/2020

Date Created:

2019-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:14701 and etdadmin_upload_667038

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Using SAMDI-MS and Peptide Arrays to Study Protein Tyrosine Phosphatases and Discover New Cellular Regulatory Mechanisms

Applications of SAMDI Mass Spectrometry for High-Throughput Reaction Monitoring on Peptide Arrays

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