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Description:: High-throughput methods enable rapid experimentation and/or screening of thousands of samples simultaneously. Mass-spectrometry based methods are of particular interest since they provide a label-free way to detect all species present in a given reaction mixture. To circumvent sample preparation and purification—which is typically a slow process—the Mrksich group developed a...
Keyword:: SAMDI, Biochemical Assays, High-Throughput, Single-Cell, and Mass Spectrometry
Subject:: Chemistry
Creator:: Hakim Moully, Elamar
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 10/06/2021
Date Created:: 2021-01-01
Resource Type:: Dissertation
Alternate Identifier:: http://dissertations.umi.com/northwestern:15661 and etdadmin_upload_836023

Description:: Self-Assembled Monolayers for MALDI-TOF Mass Spectrometry (SAMDI-MS) is a technique that combines self-assembling molecules of alkane disulfides on gold and MALDI-TOF mass spectrometry. By using well-defined monolayers with functionalizations that both prevent non-specific adsorption onto the surface and presents immobilization handles, it is possible to pull out analytes of interest,...
Keyword:: Peptides, Data Storage, MALDI, and SAMDI
Subject:: Chemistry
Creator:: Ten, Alexei Sergeevich
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 04/29/2020
Date Created:: 2020-01-01
Resource Type:: Dissertation
Alternate Identifier:: etdadmin_upload_718764 and http://dissertations.umi.com/northwestern:15001

Description:: Label-free assays, and particularly those based on the combination of mass spectroscopy with surface chemistries, enable high-throughput experiments of a broad range of reactions. However, these methods can still require the incorporation of functional groups that allow immobilization of reactants and products to surfaces prior to analysis. In this thesis,...
Keyword:: Nanotechnology, SAMDI, Surface chemistry, Microarray, Mass Spectrometry, and Reactions
Subject:: Chemistry, Biomedical engineering, and Chemical engineering
Creator:: Helal, Kazi Yasin
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 04/28/2020
Date Created:: 2019-01-01
Resource Type:: Dissertation
Alternate Identifier:: http://dissertations.umi.com/northwestern:15011 and etdadmin_upload_719318

Description:: This work combines the use of high-throughput mass spectrometry with peptide arrays for to monitor reactions on peptides. The Mrksich lab introduced a high-throughput, label-free, biochemical assay that relies on self-assembled monolayers on gold and matrix-assisted laser desorption/ionization mass spectrometry, termed SAMDI-MS. This dissertation introduces new applications of SAMDI-MS and...
Keyword:: High-throughput, SAMDI, Phosphatase, Mass spectrometry, and Peptide Arrays
Subject:: Chemistry and Biochemistry
Creator:: Szymczak, Lindsey Catherine
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 02/12/2020
Date Created:: 2019-01-01
Resource Type:: Dissertation
Alternate Identifier:: http://dissertations.umi.com/northwestern:14701 and etdadmin_upload_667038

Description:: SAMDI-MS (Self-Assembled Monolayers for MALDI-TOF Mass Spectrometry) couples the use of chemically-defined self-assembled monolayers of alkane thiolates on gold surfaces with MALDI-TOF mass spectrometry for rapid characterization of the surfaces. Reactions performed on the surfaces can be quantified directly by detection of the reaction substrates and products. This rapid detection...
Keyword:: SAMDI, mass spectrometry, bioassay, chromodomains, cell-free reactions, and microfluidics
Subject:: Analytical chemistry, Materials Science, and Biochemistry
Creator:: O'Kane, Patrick Thomas
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 02/12/2020
Date Created:: 2019-01-01
Resource Type:: Dissertation
Alternate Identifier:: http://dissertations.umi.com/northwestern:14615 and etdadmin_upload_661860

Description:: More than half of proteins in humans are modified with carbohydrates in a process called glycosylation, yet this process remains poorly understood even though approximately 1% of the expressed human genome encodes biosynthetic machinery for glycosylation. Unlike genomics and proteomics where high throughput tools are now routinely used to generate...
Keyword:: SAMDI, High-throughput protein synthesis, glycosylation, self-assembled monolayers, glycosyltransferase, and Mass spectrometry
Subject:: Bioengineering, Analytical chemistry, and Biochemistry
Creator:: Techner, Jose-Marc
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 02/12/2020
Date Created:: 2019-01-01
Resource Type:: Dissertation
Alternate Identifier:: http://dissertations.umi.com/northwestern:14598 and etdadmin_upload_661107

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