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Cell-free Platforms for Synthesis of Non-standard Polypeptides in vitro

Description:

Proteins represent a critical class of biomolecules, universally employed by all living organisms to fulfill essential structural, functional, and enzymatic roles necessary to support life. In nature, these polymers are composed generally of twenty natural amino acid (AA) building blocks, which can be modified with covalent adducts known as post-translational...

Keyword:

post-translational modification, genome engineering, cell-free protein synthesis, synthetic biology, orthogonal translation system, and non-canonical amino acid

Subject:

Bioengineering, Biology, and Biochemistry

Creator:

Des Soye, Benjamin James

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

02/12/2020

Date Created:

2018-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:14473 and etdadmin_upload_627384
Establishing Novel Cell-free Platforms for the Synthesis of Protein-based Biologics

Description:

There is a need for alternative biomanufacturing solutions to produce novel medicines and help solve long-standing issues regarding difficult-to-treat diseases. Cell-free protein synthesis (CFPS) provides scientists with a new paradigm for producing proteins with an unprecedented freedom and control over the biomolecular machinery involved. In this document, I describe my...

Keyword:

antibodies, genome engineering, non-canonical amino acid, synthetic biology, and Cell-free protein synthesis

Subject:

Chemical and Biological Engineering

Creator:

Rey William Martin

Owner:

Scholarly Digital Publishing

Date Uploaded:

10/21/2018

Date Modified:

10/21/2018

Date Created:

2017-01-01

Resource Type:

Dissertation

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