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High-Throughput Development of Glycosyltransferase Assays Using SAMDI-MS

Description:

More than half of proteins in humans are modified with carbohydrates in a process called glycosylation, yet this process remains poorly understood even though approximately 1% of the expressed human genome encodes biosynthetic machinery for glycosylation. Unlike genomics and proteomics where high throughput tools are now routinely used to generate...

Keyword:

SAMDI, High-throughput protein synthesis, glycosylation, self-assembled monolayers, glycosyltransferase, and Mass spectrometry

Subject:

Bioengineering, Analytical chemistry, and Biochemistry

Creator:

Techner, Jose-Marc

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

02/12/2020

Date Created:

2019-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:14598 and etdadmin_upload_661107
Using Mass Spectrometry and Self-Assembled Monolayers for Applications in Biochips

Description:

Biochips and high-throughput screening methods are powerful tools for studying biological processes. The combination of peptide arrays and SAMDI mass spectrometry incorporates these tools to study a broad range of biochemical transformations, including enzyme activities, to provide valuable information on substrate recognition and guide the development of diagnostic and therapeutic...

Keyword:

high-throughput screening, enzyme activity, peptides, self-assembled monolayers, chemical biology, and biochips

Subject:

Bioengineering

Creator:

Wood, Sarah Elizabeth

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

02/12/2020

Date Created:

2019-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:14669 and etdadmin_upload_662973

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High-Throughput Development of Glycosyltransferase Assays Using SAMDI-MS

Using Mass Spectrometry and Self-Assembled Monolayers for Applications in Biochips

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