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- Description:
- This dissertation focuses on quantifying protein folding stability determinants and presenting initial experiments that can guide the development of a novel assay that identifies cell-penetrating miniproteins. First, despite over a century of scholarship on protein folding stability, applying this knowledge to design proteins computationally remains limited. Usually, protein designers generate...
- Keyword:
- protein design, cell-penetrating protein, protein folding stability, and synthetic biology
- Subject:
- Biology, Cellular biology, and Biophysics
- Creator:
- Kim, Tae-Eun
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 08/23/2023
- Date Created:
- 2023-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_1008136 and http://dissertations.umi.com/northwestern:16683
-
- Description:
- This dissertation focuses on quantifying protein folding stability determinants and presenting initial experiments that can guide the development of a novel assay that identifies cell-penetrating miniproteins. First, despite over a century of scholarship on protein folding stability, applying this knowledge to design proteins computationally remains limited. Usually, protein designers generate...
- Keyword:
- protein design, cell-penetrating protein, synthetic biology, and protein folding stability
- Subject:
- Biology, Cellular biology, and Biophysics
- Creator:
- Kim, Tae-Eun
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 08/23/2023
- Date Created:
- 2023-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:16695 and etdadmin_upload_1009800
-
- Description:
- By 2030, up to half of the world’s population is projected to suffer from water insecurity: a chronic scarcity of potable water due to rapidly warming temperatures, increased agricultural demand, and pollution. The health impacts of contaminated water are profound: hundreds of thousands of global deaths each year are ascribed...
- Keyword:
- diagnostics, water quality, cell-free, and synthetic biology
- Subject:
- Bioengineering, Chemical engineering, and Public health
- Creator:
- Silverman, Adam Daniel
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/28/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15551 and etdadmin_upload_817796
-
- Description:
- The need to rapidly develop and produce life-saving vaccines and therapeutics is critical for overcoming pandemics in a global economy. Recent advances in automation and cell-free systems have opened new avenues for expediting optimization and production of biologic vaccines and therapeutics. A key consideration for the development of protein biologics...
- Keyword:
- biotechnology, bioproduction, glycosylation, vaccines, cell-free protein synthesis, and synthetic biology
- Subject:
- Chemical engineering
- Creator:
- Hershewe, Jasmine Marie
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/01/2021
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_780613 and http://dissertations.umi.com/northwestern:15396
-
- Description:
- This dissertation focuses on the development of quantitative approaches for characterizing endogenous signaling pathways and designing new pathways in mammalian cells. I demonstrate how mathematical descriptions that are formulated to explain gene expression patterns can also serve as a powerful springboard for deeper analyses into the properties and functions of...
- Keyword:
- macrophage, systems biology, receptor, computational biology, transcription factor, and synthetic biology
- Subject:
- Bioengineering, Molecular biology, and Immunology
- Creator:
- Muldoon, Joseph Jacob
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/01/2021
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15256 and etdadmin_upload_764084
-
- Keyword:
- transcription, aptamer, RNA polymerase III, RNA, synthetic biology, and termination
- Creator:
- Matthew Verosloff, Dr. Julius Lucks, David Bushhouse, Dr. Joshua Leonard, William Corcoran, and Taylor Dolberg
- Owner:
- Matthew Stuart Verosloff
- Date Uploaded:
- 09/09/2020
- Date Modified:
- 02/15/2021
- Resource Type:
- Dataset
-
- Description:
- Since their introduction nearly a century ago, protein vaccines and therapeutics have revolutionized our ability to prevent and treat human disease. However, existing production processes for biopharmaceuticals are technically complex and rely on living cells, which necessitates highly centralized manufacturing in large-scale production facilities, specialized equipment, and cold-chain distribution. With...
- Keyword:
- glycoprotein therapeutics, cell-free protein synthesis, decentralized biomanufacturing, synthetic biology, STEM education, and conjugate vaccines
- Subject:
- Chemical engineering
- Creator:
- Stark, Jessica C
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Modified:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_686044 and http://dissertations.umi.com/northwestern:14826
-
- Description:
- Proteins represent a critical class of biomolecules, universally employed by all living organisms to fulfill essential structural, functional, and enzymatic roles necessary to support life. In nature, these polymers are composed generally of twenty natural amino acid (AA) building blocks, which can be modified with covalent adducts known as post-translational...
- Keyword:
- post-translational modification, genome engineering, cell-free protein synthesis, synthetic biology, orthogonal translation system, and non-canonical amino acid
- Subject:
- Bioengineering, Biology, and Biochemistry
- Creator:
- Des Soye, Benjamin James
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2018-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14473 and etdadmin_upload_627384
-
- Description:
- It is pertinent now more than ever that we find sustainable alternatives to produce chemicals. For decades, scientists and engineers have turned to biological systems to help meet societal needs in energy, medicine, materials, and moreespecially when chemical synthesis is untenable. Often, biologically-produced small molecules are insufficient for production at...
- Keyword:
- metabolic engineering, biotechnology, cell-free systems, biosynthetic pathway, synthetic biology, and enzymatic reactions
- Subject:
- Chemical and Biological Engineering
- Creator:
- Ashty Karim
- Owner:
- Scholarly Digital Publishing
- Date Uploaded:
- 10/14/2019
- Date Modified:
- 11/20/2019
- Date Created:
- 2018-01-01
- Resource Type:
- Dissertation
-
- Description:
- Recent advances in cell-free synthetic biology have spurred the development of in vitro molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However, cell-free sensors for detecting manmade organic water contaminants such as pesticides are sparse, partially because few characterized natural biological sensors can directly detect such pollutants. Here,...
- Keyword:
- biosensor, TX-TL, atrazine, cell-free, cyanuric acid, metabolism, synthetic biology, and transcription factor
- Creator:
- Umut Akova, Khalid K. Alam, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman
- Owner:
- Adam Silverman
- Date Uploaded:
- 09/23/2019
- Date Modified:
- 02/19/2020
-
- Description:
- Metabolic engineering of microorganisms to produce useful compounds from renewable substrates is a promising means for sustainable, on-demand production of chemicals. However, efforts to design and engineer microbial cell factories are constrained by costly and slow “build” times in which each genetic variation requires re-engineering a new strain for each...
- Keyword:
- metabolic engineering, cell-free, synthetic biology, prototyping, and limonene
- Subject:
- Chemical and Biological Engineering
- Creator:
- Quentin Merle Dudley
- Owner:
- Scholarly Digital Publishing
- Date Uploaded:
- 01/25/2019
- Date Modified:
- 01/30/2019
- Date Created:
- 2017-01-01
- Resource Type:
- Dissertation
-
- Description:
- There is a need for alternative biomanufacturing solutions to produce novel medicines and help solve long-standing issues regarding difficult-to-treat diseases. Cell-free protein synthesis (CFPS) provides scientists with a new paradigm for producing proteins with an unprecedented freedom and control over the biomolecular machinery involved. In this document, I describe my...
- Keyword:
- antibodies, genome engineering, non-canonical amino acid, synthetic biology, and Cell-free protein synthesis
- Subject:
- Chemical and Biological Engineering
- Creator:
- Rey William Martin
- Owner:
- Scholarly Digital Publishing
- Date Uploaded:
- 10/21/2018
- Date Modified:
- 10/21/2018
- Date Created:
- 2017-01-01
- Resource Type:
- Dissertation
-
- Description:
- A longstanding goal of synthetic biology has been the programmable control of cellular functions. Central to this is the creation of versatile regulatory toolsets that allow for programmable control of gene expression. Of the many regulatory molecules available, RNA regulators offer the intriguing possibility of de novo design – allowing...
- Keyword:
- transcription, RNA, RNA engineering, synthetic biology, and gene regulation
- Subject:
- Synthetic Biology and RNA engineering
- Creator:
- Matthew Verosloff, Alexandra Westbrook, James Chappell, and Julius B. Lucks
- Owner:
- Julius Beau Lucks
- Publisher:
- MacMillan Publishers
- Language:
- English
- Date Uploaded:
- 08/03/2017
- Date Modified:
- 01/07/2020
- Date Created:
- 2017-08-03
- Resource Type:
- Dataset
- Alternate Identifier:
- doi:10.21985/N28S9B