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-
- Description:
- Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are expressed in the heart and central nervous system where they regulate membrane excitability. Recently, inhibiting HCN channels in the hippocampus has been identified as a possible therapeutic intervention for the treatment of Major Depressive Disorder (MDD). However, based on the high expression level of...
- Keyword:
- HCN, OPC, Oligodendrocyte, TRIP8b, and Depression
- Subject:
- Neuroscience, Biochemistry, and Developmental Biology
- Creator:
- Kyle Andrew Lyman
- Owner:
- Scholarly Digital Publishing
- Date Uploaded:
- 06/17/2019
- Date Modified:
- 06/17/2019
- Date Created:
- 2016-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- https://doi.org/10.21985/N2W781
-
- Description:
- Proteins represent a critical class of biomolecules, universally employed by all living organisms to fulfill essential structural, functional, and enzymatic roles necessary to support life. In nature, these polymers are composed generally of twenty natural amino acid (AA) building blocks, which can be modified with covalent adducts known as post-translational...
- Keyword:
- post-translational modification, genome engineering, cell-free protein synthesis, synthetic biology, orthogonal translation system, and non-canonical amino acid
- Subject:
- Bioengineering, Biology, and Biochemistry
- Creator:
- Des Soye, Benjamin James
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2018-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14473 and etdadmin_upload_627384
-
- Description:
- Methylation of histone 3 lysine 4 (H3K4) catalyzed by the COMPASS family of lysine methyltransferases is universally associated with eukaryotic transcription. However, despite thousands of published studies examining the deposition, dynamics, and genomic positions of this chromatin modification, there is no clear consensus as to the molecular function or biological...
- Subject:
- Biochemistry
- Creator:
- Rickels, Ryan A
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14787 and etdadmin_upload_682860
-
- Description:
- This work combines the use of high-throughput mass spectrometry with peptide arrays for to monitor reactions on peptides. The Mrksich lab introduced a high-throughput, label-free, biochemical assay that relies on self-assembled monolayers on gold and matrix-assisted laser desorption/ionization mass spectrometry, termed SAMDI-MS. This dissertation introduces new applications of SAMDI-MS and...
- Keyword:
- High-throughput, SAMDI, Phosphatase, Mass spectrometry, and Peptide Arrays
- Subject:
- Chemistry and Biochemistry
- Creator:
- Szymczak, Lindsey Catherine
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14701 and etdadmin_upload_667038
-
- Description:
- The work in this thesis focuses on computational methods for the identification of novel enzymatic pathways. In particular this work focuses on the utilization of the Biological Network Integrated Computational Explorer (BNICE) software suite to predict de novo enzymatic pathways for the production of commercially relevant compounds and on improvements...
- Subject:
- Bioengineering and Biochemistry
- Creator:
- Stine, Andrew Eugene
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2016-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_614431 and http://dissertations.umi.com/northwestern:14393
-
- Description:
- SAMDI-MS (Self-Assembled Monolayers for MALDI-TOF Mass Spectrometry) couples the use of chemically-defined self-assembled monolayers of alkane thiolates on gold surfaces with MALDI-TOF mass spectrometry for rapid characterization of the surfaces. Reactions performed on the surfaces can be quantified directly by detection of the reaction substrates and products. This rapid detection...
- Keyword:
- SAMDI, mass spectrometry, bioassay, chromodomains, cell-free reactions, and microfluidics
- Subject:
- Analytical chemistry, Materials Science, and Biochemistry
- Creator:
- O'Kane, Patrick Thomas
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14615 and etdadmin_upload_661860
-
- Description:
- Epigenetics is the study of chromatin-based events that regulate gene expression without the change of DNA sequence, including DNA methylation, histone modification and chromatin remodeling. Epigenetic regulators are encoded to modify chromatin in a highly regulated and dynamic manner. A growing number of studies have suggested the dysregulation of epigenetic...
- Keyword:
- Biological sciences, Histone methylation, Proteomics, Mass spectrometry, Protein interaction, and Epigenetics
- Subject:
- Analytical chemistry, Molecular biology, and Biochemistry
- Creator:
- Huang, Xiaoxiao
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_686355 and http://dissertations.umi.com/northwestern:14838
-
- Description:
- More than half of proteins in humans are modified with carbohydrates in a process called glycosylation, yet this process remains poorly understood even though approximately 1% of the expressed human genome encodes biosynthetic machinery for glycosylation. Unlike genomics and proteomics where high throughput tools are now routinely used to generate...
- Keyword:
- SAMDI, High-throughput protein synthesis, glycosylation, self-assembled monolayers, glycosyltransferase, and Mass spectrometry
- Subject:
- Bioengineering, Analytical chemistry, and Biochemistry
- Creator:
- Techner, Jose-Marc
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14598 and etdadmin_upload_661107
-
- Description:
- The literature has established glucokinase (GCK) to be the principal hexokinase (HK) in the liver, operating as a glucose sensor to regulate glucose metabolism and lipid homeostasis. We have recently proposed Hexokinase Domain Containing-1 (HKDC1) to be a novel 5th HK with expression in the liver. Here, we reveal HKDC1...
- Keyword:
- Hexokinase, NAFLD, Liver Metabolism, and HKDC1
- Subject:
- Molecular biology, Biology, and Biochemistry
- Creator:
- Pusec, Carolina
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2018-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14450 and etdadmin_upload_626205
-
- Description:
- One of the grand challenges in science is development of soft materials that mimic living organisms to optimize the way we use energy, translate or morph reversibly or sense their envi- ronment and respond in a useful fashion. Using the insights from studying biological structures, we hope to design soft...
- Subject:
- Materials Science, Biochemistry, and Computational chemistry
- Creator:
- Iscen, Aysenur
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14770 and etdadmin_upload_679718
-
- Description:
- Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas proteins provide an immune-like response in many prokaryotes against extraneous nucleic acids. CRISPR-Cas systems are classified into different classes and types that vary widely in composition, target recognition, and overall mechanism. The main division of CRISPR-Cas systems occurs between...
- Keyword:
- Staphylococcus epidermidis, X-ray crystallography, Cryo-EM, Csm3, CRISPR, and Csm2
- Subject:
- Molecular biology, Biochemistry, and Biophysics
- Creator:
- Dorsey, Bryan William
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_655609 and http://dissertations.umi.com/northwestern:14567
-
- Description:
- Harnessing the metabolic potential of methanotrophic bacteria is a compelling strategy for the bioremediation of environmentally harmful methane gas. Methanotrophs can activate a 105 kcal/mol C-H bond in methane at ambient conditions using metalloenzymes called methane monooxygenases (MMOs). Particulate methane monooxygenase (pMMO) is a copper-dependent, membrane-bound enzyme that is the...
- Keyword:
- copper, methane, methanotroph, metalloenzyme, particulate methane monooxygenase, and membrane protein
- Subject:
- Microbiology, Chemistry, and Biochemistry
- Creator:
- Ro, Soo Yeon
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/12/2020
- Date Created:
- 2019-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14705 and etdadmin_upload_667719
-
- Description:
- Nucleic acid drugs promise to revolutionize the development of therapeutics. They offer a platform for digital medicine, where systematic changes to the nucleic acid sequence can be utilized to target the entire human genome. However, nucleic acids suffer from a number of drawbacks, such as negligible cellular uptake and rapid...
- Keyword:
- siRNA, Spherical Nucleic Acids, and High Throughput Analysis
- Subject:
- Nanotechnology, Nanoscience, and Biochemistry
- Creator:
- Yamankurt, Gokay
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 04/29/2020
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:14982 and etdadmin_upload_717353
-
- Description:
- Natural products from fungi have had an immeasurable societal impact as both friends and foes to human health. This includes numerous therapeutics that have had widespread clinical success, mycotoxins that are implicated in fungal pathogenicity, and various agrochemicals. Recent genome sequencing efforts have revealed that fungal genomes contain the capacity...
- Subject:
- Biochemistry
- Creator:
- Robey, Matthew
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 01/21/2021
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_731063 and http://dissertations.umi.com/northwestern:15039
-
- Description:
- The interaction of amyloid-β (Aβ) with endogenous metal ions is thought to play a role in the pathogenesis of Alzheimer’s disease (AD). However, limited tools exist to study and modulate Aβ-metal binding. The Meade lab has developed cobalt(III) Schiff base (Co(III)-sb) complexes as protein inhibitors that competitively displace metals from...
- Keyword:
- amyloid-beta, aggregation, cobalt(III) Schiff base, and coordination complex
- Subject:
- Biology, Biochemistry, and Neurosciences
- Creator:
- Roberts, Kaleigh Filisa
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/01/2021
- Date Created:
- 2018-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_773649 and http://dissertations.umi.com/northwestern:15349
-
- Description:
- Although centrosomes nucleate and organize microtubules in mitotically-dividing cells, spindles in female reproductive cells (oocytes) form in their absence. In some organisms acentrosomal spindle assembly is mediated by acentriolar microtubule organizing centers (MTOCs) that are thought to functionally replace centrosomes. However, spindle assembly in human oocytes does not require MTOCs;...
- Keyword:
- acentrosomal, meiosis, acentriolar, kinesin, microtubule, and C. elegans
- Subject:
- Molecular biology, Biochemistry, and Cellular biology
- Creator:
- Wolff, Ian
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/01/2021
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_763687 and http://dissertations.umi.com/northwestern:15248
-
- Description:
- Heart failure due to genetic cardiomyopathy is associated with a range of phenotypic expression. The studies in this body of work interrogated the role of noncoding variation in modifying cardiomyopathy phenotypes. We used cap analysis of gene expression in heathy and failed left ventricles to define the regulatory environment of...
- Keyword:
- noncoding, genetics, MYH7, heart failure, modifier, and enhancer
- Subject:
- Molecular biology, Genetics, and Biochemistry
- Creator:
- Gacita, Anthony Martin
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/01/2021
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_756135 and http://dissertations.umi.com/northwestern:15193
-
- Description:
- Proper partitioning of mitochondria and mtDNA is critical for cellular health. Investigations into mitochondrial inheritance, specifically how mtDNA inheritance is coupled with the inheritance mitochondrial compartment, are still in the early stages. We use budding yeast as a model polarized cell system to study a mitochondrial Myo2-adaptor protein, Mmr1, in...
- Keyword:
- mitochondria, mitochondrial tethering, mtDNA, Mmr1, and mitochondrial inheritance
- Subject:
- Molecular biology, Biochemistry, and Cellular biology
- Creator:
- Chen, WeiTing
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/01/2021
- Date Created:
- 2020-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_763646 and http://dissertations.umi.com/northwestern:15245
-
- Description:
- Melanin is a functional biopolymer most commonly associated with human skin pigmentation, where it serves as a radiation protection agent, shielding us from the harmful effects of UV radiation. However, melanin is also present in human ears, eyes, hair, and brains, serving a variety of functions. In fact, melanin...
- Keyword:
- porosity, toxin adsorption, nanoparticles, fungal, melanin, and radiation protection
- Subject:
- Materials Science and Biochemistry
- Creator:
- McCallum, Naneki C.
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 04/15/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15478 and etdadmin_upload_797429
-
- Description:
- Transcription of nucleic acids (DNA and RNA) is a hallmark of life, taking the information stored within genomic nucleic acids and converting it into a form that is useful for producing the proteins necessary for cellular and organismal function. In eukaryotes, transcription of DNA into messenger RNA (mRNA) requires the...
- Keyword:
- Virology, Paramyxovirus, Cryo-EM, Mediator, Polymerase, and Transcription
- Subject:
- Molecular biology, Biochemistry, and Biophysics
- Creator:
- Abdella, Ryan
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 04/15/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15490 and etdadmin_upload_797897
-
- Description:
- Meiosis is a highly regulated process necessary for proper chromosome division. Zincfluxes regulate mammalian meiosis; between prophase I and metaphase II, total intracellular zinc increases by 50%, while 20% of zinc is released in “zinc sparks” following fertilization. Although zinc fluxes had been shown to be conserved in mammals, it...
- Keyword:
- Xenopus, Sample Fixation, Zinc, Manganese, Fertilization, and EMI2
- Subject:
- Inorganic chemistry, Biology, and Biochemistry
- Creator:
- Seeler, John Frederick
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/28/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_819316 and http://dissertations.umi.com/northwestern:15573
-
- Description:
- The opposing activities of phosphatases and kinases determine the phosphorylation status of proteins, yet kinases have received disproportionate attention in studies of cellular processes, with the roles of phosphatases remaining less understood. This dissertation describes the use of self-assembled monolayer laser desorption/ionization mass spectrometry (SAMDI-MS) together with peptide arrays to...
- Keyword:
- SAMDI-MS, Phosphatases, High-Throughput Experiments, Enzyme Specificity, β-Catenin, and Peptide Arrays
- Subject:
- Chemistry, Biochemistry, and Cellular biology
- Creator:
- Huang, Che-Fan
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/28/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_819016 and http://dissertations.umi.com/northwestern:15570
-
- Description:
- The treatment of AML remains to be a challenge due to the high rates of resistance and relapse experienced by patients after initial therapy. The MAPK-interacting kinases 1 and 2 (MNK1/2) have generated increasing interest as therapeutic targets for AML due to their critical role in malignant hematopoietic transformation via...
- Subject:
- Molecular biology, Biology, and Biochemistry
- Creator:
- Suarez Palacios, Milagros Melanie
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 10/07/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_845430 and http://dissertations.umi.com/northwestern:15772
-
- Description:
- This thesis proposes a robust multi-pronged approach to study the effect of nanoparticles on cells. In the first place, this work is focused on investigation of the protein corona that accumulates on the surface of nanoparticles internalized by the cells and their poly-pathway effects on protein availability and messenger RNA...
- Keyword:
- PARP, Nanoparticles, BIRC5, XFM, and SR-FTIR
- Subject:
- Nanotechnology, Biology, and Biochemistry
- Creator:
- Lastra, Ruben Omar
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 10/07/2021
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_837951 and http://dissertations.umi.com/northwestern:15671
-
- Description:
- Cobalt(III) Schiff-base complexes (Co(III)-sb) have been utilized in the literature as antibacterial, antiviral, and inhibitory agents. Recent work has utilized their ability to displace endogenous metals from metalloproteins that exhibit aberrant gain of function pathologies in human disease. Specifically, in this dissertation Co(III)-sb has been applied as inhibitors of pathogenic...
- Keyword:
- Transcription Factors, Coordination Complexes, Cobalt, Amyloid-beta, Molecular Dynamics, and Intrinsically Disordered Proteins
- Subject:
- Chemistry, Biochemistry, and Biophysics
- Creator:
- Brue, Christopher Robert
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/02/2022
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15862 and etdadmin_upload_863221
-
- Description:
- The eukaryotic genome is packaged into chromatin. The nucleosome, the basic unit of chromatin, is composed of DNA coiled around a histone octamer. Histones are among the longest-lived protein species in mammalian cells, due to their thermodynamic stability and their associations with DNA and histone chaperones. Histone metabolism plays an...
- Keyword:
- UBR7, NASP, histone, H3, chaperone, and chromatin
- Subject:
- Biology, Biochemistry, and Genetics
- Creator:
- Ferguson, Ann
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/02/2022
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15808 and etdadmin_upload_853746
-
- Description:
- T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease, affecting children and adults. Chemotherapy regimens show high response rates but have debilitating effects and carry risk of relapse. Until now, no targeted therapy has been approved. In addition, 40% of patients will relapse and their treatment options are limited because...
- Keyword:
- USP11, LCK, USP7, glucocorticoid, leukemia, and ubiquitination
- Subject:
- Molecular biology, Oncology, and Biochemistry
- Creator:
- Gutierrez Diaz, Blanca Teresa
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/02/2022
- Date Created:
- 2021-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_862777 and http://dissertations.umi.com/northwestern:15858
-
- Description:
- SAMDI-MS, which stands for self-assembled monolayers (SAMs) for matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS), is a powerful tool that has enabled the development of novel high-throughput screening and experimentation methods for decades. SAMDI-MS works by immobilizing analytes to functionalized SAMs prior to MS analysis and is capable of...
- Subject:
- Bioengineering, Molecular biology, and Biochemistry
- Creator:
- Pluchinsky, Adam John
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_885633 and http://dissertations.umi.com/northwestern:15968
-
- Description:
- Methanotrophs, bacteria that can metabolize methane, remain a promising solution to mitigating the effects of climate change by removing atmospheric methane and converting it to useful chemical precursors. However, a full understanding of the main enzyme they use to oxidize methane, particulate methane monooxygenase (pMMO), is critical for harnessing their...
- Keyword:
- cryogenic electron tomography, particulate methane monooxygenase, membrane protein, cryogenic electron microscopy, and cell-free protein synthesis
- Subject:
- Molecular biology, Biochemistry, and Biophysics
- Creator:
- Koo, Christopher Wellington
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_897671 and http://dissertations.umi.com/northwestern:16003
-
- Description:
- Heat shock factor 1 (HSF1) is well known for its role in the heat shock response (HSR), where it drives a transcriptional program comprising heat shock protein (HSP) genes, and in tumorigenesis, where it drives a program comprising HSPs and many noncanonical target genes that support malignancy. Here, we find...
- Subject:
- Biochemistry
- Creator:
- Smith, Roger Stephen
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_902929 and http://dissertations.umi.com/northwestern:16081
-
- Description:
- MYC regulates multiple gene programs, raising questions about the potential selectivity and downstream transcriptional consequences of MYC inhibitors as cancer therapeutics. MYC func-tions to either globally amplify RNA production or selectively regulates genes by repression or ac-tivation. In models of MYC inhibition by small molecules the functionality of MYC is...
- Subject:
- Molecular biology and Biochemistry
- Creator:
- Holmes, Austin Gable
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_904142 and http://dissertations.umi.com/northwestern:16111
-
- Description:
- DNA double-strand breaks (DSBs) are caused by either endogenous agents or exogenous ionizing radiation and chemicals. Incorrect DSB repair can lead to undesired genome rearrangements. Homologous recombination (HR) and non-homologous end-joining (NHEJ) pathways are two major DNA repair pathways that repair DSBs and maintain genome integrity. When homologous DNA is...
- Keyword:
- DNA repair, Cryo EM, NHEJ, DNA-PKcs, Cancer, and CRISPR
- Subject:
- Molecular biology, Biochemistry, and Biophysics
- Creator:
- Chen, Siyu
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_879511 and http://dissertations.umi.com/northwestern:15936
-
- Description:
- Eukaryotic genomes are organized into chromatin, which acts to regulate access to the organism’s genetic material. A large and diverse class of proteins, known as chromatin modifiers and remodelers, are responsible for regulating the composition and structure of chromatin by monitoring nucleosomes. Chromatin remodelers are involved in multiple cellular processes,...
- Subject:
- Biology, Biochemistry, and Biophysics
- Creator:
- Reyes, Alexis Amaris
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 06/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:15930 and etdadmin_upload_879245
-
- Description:
- Transfer of information across membranes is fundamental to the function of all organisms and is primarily initiated by transmembrane receptors. This is an allosteric process and involves conformational coupling between ligand-binding domain and signaling domain of a receptor. This allosteric mechanism of activation is unclear for many receptors. Moreover, for...
- Keyword:
- GPCR, CaSR, and smFRET
- Subject:
- Bioinformatics, Biochemistry, and Biophysics
- Creator:
- Schamber, Michael Robert
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 09/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:16268 and etdadmin_upload_929368
-
- Description:
- Breakthroughs in large-scale biological data collection have resulted in a wealth of -omics (genomics, metabolomics, etc.) datasets in the literature. However, the development of appropriate computational techniques for their analysis is lacking, yet crucial for fully extracting the rich information contained in these datasets. The work in this dissertation describes...
- Keyword:
- Biorecording, Data Storage, Cheminformatics, Metabolomics Annotation, Machine Learning, and TdT
- Subject:
- Bioengineering, Bioinformatics, and Biochemistry
- Creator:
- Strutz, Jonathan
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 09/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_911481 and http://dissertations.umi.com/northwestern:16126
-
- Description:
- Nuclear receptors (NRs) are an important family of transcription factors that often regulate genes in response to ligands and by way of direct interactions with coactivator proteins. Many NR-coactivator pairs have been identified that cooperate to regulate transcription but fully understanding how NRs recruit specific coactivators involves learning which of...
- Keyword:
- Transcriptional Regulation, NMR, and Nuclear Receptors
- Subject:
- Biology, Biochemistry, and Biophysics
- Creator:
- Daffern, Nicolas
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 09/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:16121 and etdadmin_upload_910580
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- Description:
- The worldwide community of patients affected by Basal Cell Carcinoma of the skin (BCC) is larger than that of any other cancer. While BCC is rarely lethal, currently available treatment strategies often leave patients with disfiguring scars on their faces, heads, and necks. Moreover, the high recurrence rates of BCC...
- Keyword:
- zinc finger transcription factors, 3D skin cancer model, Basal Cell Carcinoma, Gli transcription factors, cobalt-Schiff base, and graphene oxide
- Subject:
- Inorganic chemistry, Biology, and Biochemistry
- Creator:
- Dukes, Meghan Ward
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 09/22/2022
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_923011 and http://dissertations.umi.com/northwestern:16188
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- Description:
- G protein-coupled receptors (GPCRs) are the largest family of membrane receptors in humans and play a role in nearly all physiological processes. Among GPCRs, metabotropic glutamate receptors (mGluRs) are a structurally distinct family of synaptic receptors that are essential in regulating neurotransmission and synaptic plasticity. Due to their important regulatory...
- Keyword:
- structural dynamics, mGluRs, conformational dynamics, membrane proteins, smFRET, and GPCRs
- Subject:
- Pharmacology, Biochemistry, and Biophysics
- Creator:
- Liauw, Brandon Wey-Hung
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 02/16/2023
- Date Created:
- 2022-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_937523 and http://dissertations.umi.com/northwestern:16310
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- Description:
- Genetic studies have found variants in the protein-degrading autophagy-lysosomal pathway (ALP) to be among the most common risk factors for developing Parkinson’s disease (PD). Macroautophagy (MA) is the arm of this pathway which delivers cytosolic components to lysosomes for degradation and is essential for neuronal health. The defining pathological protein...
- Keyword:
- ykt6, synuclein, Parkinson disease, SNARE, autophagy, and farnesyltransferase inhibitor
- Subject:
- Molecular biology, Biochemistry, and Neurosciences
- Creator:
- Pitcairn, Caleb
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 05/31/2023
- Date Created:
- 2023-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:16447 and etdadmin_upload_970290
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- Description:
- Stimulation of the cGAS-STING (cycle GMP-AMP synthase-Stimulator of Interferon Genes) pathway increases T cell activation and tracking into the tumor and reverses the immunosuppressive phenotype of myeloid cells. Direct targeting of the STING receptor using synthetic cyclic dinucleotide (CDN) ligands represents an attractive immunotherapeutic strategy for the treatment of lymphocyte-depleted...
- Keyword:
- GBM, Spherical nucleic acids, cGAS, Immunotherapy, and STING
- Subject:
- Biology, Biochemistry, and Immunology
- Creator:
- Mahajan, Akanksha Sanjay
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 05/31/2023
- Date Created:
- 2023-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- etdadmin_upload_983546 and http://dissertations.umi.com/northwestern:16516
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- Description:
- Pediatric high-grade gliomas (pHGGs) are aggressive pediatric CNS tumors and an important subset are characterized by mutations in H3F3A, the gene that encodes Histone H3.3 (H3.3). Substitution of Glycine at position 34 of H3.3 with either Arginine or Valine (H3.3G34R/V), was recently described and characterized in a large cohort...
- Keyword:
- genetics, G34R, cancer, ATRX, glioma, and AVB500
- Subject:
- Molecular biology, Biology, and Biochemistry
- Creator:
- Abdallah, Aalaa Sanad
- Owner:
- Scholarly Digital Publishing
- Language:
- en
- Date Uploaded:
- 05/31/2023
- Date Created:
- 2023-01-01
- Resource Type:
- Dissertation
- Alternate Identifier:
- http://dissertations.umi.com/northwestern:16475 and etdadmin_upload_978815