Using a novel DNA-microarray hybridization protocol, the diffusion distance during static microarray hybridization was estimated for Cy3- and Cy5-labeled cDNA probes as 3.8 and 2.6 mm, respectively, despite having almost identical molecular masses. Continuous mixing during microarray hybridization resulted in a 15-20% increase in signal intensity over arrays hybridized statically....
Two new methods have been developed employing silicon-containing molecules in novel organic transformations. The first strategy utilizes Lewis base-activation of triethoxysilylalkynes to deliver mild acetylide nucleophile equivalents. The second approach involves the use of enolate additions to acylsilanes to generate β-silyloxy homoenolate intermediates.
Lewis base-catalyzed activation of triethoxysilylalkynes promotes the...
In a highly competitive market, it is becoming increasingly important to view product design as an enterprise-level profit-driven activity. Demand models have become central to enterprise-driven design approaches since they help estimating the economic impact (e.g., revenues, profits, and cost) of a product's design. The primary contribution of this dissertation...
This dissertation examines the history of Mexicans' changing racial status in the Chicago metropolitan region, a place where race has traditionally been understood in strictly black and white terms. From World War I through the 1930's whites violently resisted Mexicans moving into their neighborhoods in Chicago, East Chicago, and Gary,...
This dissertation focuses on the utilization of two coordination chemistry-based synthetic approaches, which provide the ability to rapidly and quantitatively assemble sophisticated supramolecular cofacial porphyrin complexes. Significantly, by adopting a coordination chemistry-based approach, the porphyrin-porphyrin interactions can be regulated in situ via the addition or removal of chemical effector molecules....
Aluminum alloys strengthened with coherent (L1<sub>2</sub>), nanosize Al<sub>3</sub>Sc precipitates may be used as structural materials at elevated temperatures. They are creep resistant at 300°C and exhibit a threshold stress, below which creep is not measurable. Introducing ternary alloying additions, such as rare-earth elements (RE=Y, Dy, Er), that segregate within Al<sub>3</sub>Sc...
HspB2 was discovered as a chaperone of muscular dystrophy protein kinase (DMPK) and originally named, DMPK binding protein (MKBP) and presently called HspB2. Functionally, MKBP binds to DMPK and stabilizes its kinase activity, thereby accelerating the progression of muscular dystrophy. Although other binding partners of HspB2 have been found, the...
A series of investigations was undertaken to explore the processing and properties of porous titanium with 6 wt% Al and 4 wt% vanadium (Ti-6Al-4V) and an equiatomic nickel-titanium alloy (NiTi). In this study, porous materials were created in the solid state by entrapping argon in a powder compact, and subsequently...
This dissertation presents the results of three studies examining the determinants and consequences of employment among single mothers with infants and toddlers. In the first study, I use nationally-representative survey data to identify the effect of age-of-youngest-child welfare-to-work exemptions on the employment of single mothers with no more than a...
The first section of this thesis focuses on applications of DNA-modified gold nanoparticles (AuNPs) while the second part addresses the fundamental properties that arise from conjugating DNA to inorganic AuNPs. Two types of applications are discussed. The first application utilizes the colorimetric properties of AuNPs to screen duplex and triplex...
Epstein-Barr virus (EBV) is the cause of infectious mononucleosis and is associated with the etiology of several human cancers of lymphoid and epithelial cell origin, including Burkitt's lymphoma and nasopharyngeal carcinoma. It is an extremely prevalent virus in human populations worldwide. All herpesviruses, including EBV, infect cells by fusing the...
Living in a world where the machine and the Internet are ubiquitous, many people work and play online, in a world that is, ironically, often isolated and lonesome. While the Internet, as intended, connects us to information, products and services, it often draws us away from the rich connections that...
Video transmission over wireless networks to multiple mobile users has remained a challenging problem due to potential limitations on bandwidth and the time-varying nature of wireless channels. Recent advances in wireless access technologies, such as, HSDPA and IEEE 802.16, are targeted at achieving higher throughput over wireless networks. Meanwhile, advances...
The hematopoietic stem cell (HSC) niche is the site where HSCs reside in vivo. The HSC niche is formed by supporting cells that provide structure and appropriate environmental cues for HSCs. In this study, three components of the HSC niche were evaluated for their influence on HSC expansion ex vivo:...
Conventional perfusion magnetic resonance imaging with dynamic susceptibility using a contrast agents has been playing a dominant role on diagnosing a variety of cerebral nervous system disease due to lack of radiation and widespread availability. However, reliable quantification of cerebral perfusion has been elusive and challenging.
The bookend method is...
This study explored the mentoring experiences of doctoral students in science, technology, engineering, and mathematics (STEM) doctoral degree programs at Northwestern University. Research suggests that mentoring relationships support student success in STEM disciplines, and further, that lack of mentorship may be a barrier for female students. A mixed-methods approach employing...
This thesis examines the effects of micro-alloying additions to Al-Sc alloys on the microstructure, coarsening resistance and creep properties. The overarching goal of this research is to develop castable, creep-resistant aluminum alloys which can be used at temperatures in excess of 300?C. Successful high-temperature application of aluminum based alloys offers...
The macroscale mechanical and physical properties of materials are inherently linked to the underlying microstructure. Traditional continuum mechanics theories have focused on approximating the heterogeneous microstructure as a continuum, which is conducive to a partial differential equation mathematical description. Although this makes large scale simulation of material much more efficient...
This project examines the relationship between graphic design and public life in an effort to understand how graphic design functions as a mode of communication, how visual images present interpretive problems for communication scholarship, and how the increasing presence of visual images in public space inform and are informed by...
This dissertation considers price indices in the context of two rapidly growing markets. The first is the emerging market for Internet Service Providers in the mid-1990s. The second is the emergence and growth of the DVD hardware market. In both, we examine indices ranging from the most rudimentary to quality...
Across a broad spectrum of mathematical models, problems arise that have irregular domains or contain embedded interfaces. The complexity due to the introduction of these interfaces makes it more difficult to develop efficient and accurate numerical methods for their solutions.
This thesis discusses modifications to the eXtended Finite Element Method...
This dissertation argues that theatre was a vital element of postcolonial culture in Ireland in the years 1919-1932, the period in which the Irish nation emerged from revolutionary war to become a stable postcolonial state. Although critics have bemoaned the rising dominance of conservative, anti-modernist playwriting and production in Ireland's...
My dissertation explores the rational and emotional foundations of ethical decision making. Traditional research has associated ethical superiority with an analytic, rational decision making process and has suggested that emotions (e.g., empathy or anger) undermine ethics because they lead people to breach ethical principles such as the norm of impartiality....
After nearly 100 years of research, the function of sleep is unknown, prompting the desire to examine its regulation in a simpler model organism. In 2000, <em>Drosophila</em> was described as a novel model system to investigate sleep. These early studies defined features of normal sleep in <em>Drosophila</em>, and presented evidence...
Interfaces and surfaces are ubiquitous in the environment. Heterogeneous binding events and reactions control the transport and speciation of dissolved pollutants in groundwater. Many heterogeneous processes occur at mineral oxide/water interfaces, and can be controlled either by the mineral oxide, or organic molecules bound to the surface. The prevalent natural...
Child maltreatment and violence are two major public health concerns in the United States. The relationship between maltreatment and subsequent violent behavior, also referred to as the "cycle of violence", is not well understood. The present study examines whether a history of maltreatment predicts violent behavior in a sample of...
This dissertation explores price differences observed in the market. While some differences are necessitated by market conditions, the others are strategic. I illustrate the former in a study of electricity spot markets in Italy and the latter for the video rental industry.
The first project examines the welfare gains from...
The dissertation research examines the evolution of EU social and employment policy in regard to gender equality in the labor market and analyzes how EU guidelines of the European Employment Strategy (EES) and EU directives on social policy have different effects on political processes in the United Kingdom and Germany....
Musical theater is a discipline that, by its very nature, engages several disciplines, specifically music, visual art, theater, and dance. The elementary classroom is a viable setting for the study of the arts in an interdisciplinary manner, yet the number of studies examining interdisciplinary instruction with regard to elementary music...
The linear food chain of high school biology textbooks has been replaced by the food web, the increasingly complex network of trophic interactions in an ecosystem. This complexity, however, masks a number of robust statistical properties. Yet much research to date has concentrated upon predicting and testing the existence of...
In this dissertation I examine the impact of core values on foreign policy opinion, the dynamics of value change, and the differences between elites and the mass public in their values change. I find that two core values - humanitarianism and democracy - strongly affect citizens' support for various anti-terrorism...
Behavioral activation (BA) trains depressed clients to engage in more positive activities in order to increase their experience of pleasure and accomplishment, thereby reducing depression. Recent research suggested that BA might be as effective in treating depression as current leading treatments, such as cognitive-behavioral therapy (CBT) and antidepressant medication (Jacobson,...
By combining cultural theory with empirical data, this dissertation asserts that late-twentieth-century mainstream theatre had the potential to support emergent ideologies in the U.S. context. The study finds fault with those who dismiss mainstream theatre based on its commercialism and shows how a production's mainstream status may position its emergent...
This thesis presents results and analysis on a series of donor-bridge-acceptor (D-B-A) charge transfer systems in which we use time-resolved spectroscopy and magnetic resonance techniques to study the relationship of molecular structure to energy, charge, and spin transfer dynamics. We find that the sequence of events following the initial charge...
Markov models are widely employed in cost-effectiveness analysis of healthcare interventions. Although such models are usually formulated at the individual level, it is also useful to examine outcomes at the population level. Analysts may wish to know the impact of a health intervention on a whole population instead of an...
A comprehensive understanding of how image processing occurs in the primary visual cortex (V1) requires learning what aspects of neuronal responses are driven by strong feed-forward input from the lateral geniculate nucleus (LGN), and what aspects arise due to the densely recurrent network operating within the cortex itself. From an...
Although non-viral gene therapy has great potential for use in the lung, several problems including inefficient gene transfer and expression and the relative lack of cell-specific targeting have limited its applications. The two approaches that have been used to target genes to desired cell types are through specific ligand-receptor interactions...
Middle childhood is an important but often overlooked developmental period. Similarly, a large literature examines the importance of child care as a developmental context, focusing almost exclusively on pre-school child care experiences. Through three related empirical studies, this dissertation aims to address these two gaps in the extant literature. These...
The first chapter of this dissertation investigates the pricing of systematic variance risk in the equity options market. Cross sectional tests on synthetic variance swap returns reveal no evidence of a negative market variance risk premium. Furthermore, we show that a class of linear factor models cannot simultaneously explain index...
In the age of what George W. Bush has called a global democratic revolution, the freedom to consume an ever-expanding variety of images and goods in the global marketplace is often equated with the conditions of democratic freedom. With just such rhetorical elisions in mind, this dissertation examines the discursive...