Computational design of Small Transcription Activating RNAs (STARs) for versatile and dynamic gene regulation Open Access

A longstanding goal of synthetic biology has been the programmable control of cellular functions. Central to this is the creation of versatile regulatory toolsets that allow for programmable control of gene expression. Of the many regulatory molecules available, RNA regulators offer the intriguing possibility of de novo design – allowing for the bottom-up molecular-level design of genetic control systems. Here we present a computational design approach for the creation of a bacterial regulator called Small Transcription Activating RNAs (STARs) and create a library of high-performing and orthogonal STARs that achieve up to ~9000-fold gene activation. We demonstrate the versatility of these STARs – from acting synergistically with existing constitutive and inducible regulators, to reprogramming cellular phenotypes and controlling multigene metabolic pathway expression. Finally, we combine these new STARs with themselves and CRISPRi transcriptional repressors to deliver new types of RNA-based genetic circuitry that allow for sophisticated and temporal control of gene expression. Nature Communications, 2017

Descriptions

Attribute NameValues
Creator
Subject
Publisher
Language
Identifier
Keyword
Date created
Resource type
Source
  • Nature Communications
Rights
Last modified: 08/16/2017
No preview available

Download the file

Citations:

EndNote | Zotero | Mendeley

Items