Optimizing the Localized Surface Plasmon Resonance Biosensor through Self-Assembled MonolayersPublic Deposited
This paper investigates the use of self-assembled monolayers (SAMs) to optimize the localized surface plasmon resonance (LSPR) biosensor for disease detection. Various experiments were done with 1-(9-mercaptononyl)-3,6,9-trioxaundecan-11-ol (TDT) and heptaoxatricosanoic acid (HSA), a new SAM; these included a solvent study to determine refractive index sensitivity and nonspecific binding and specific binding studies to determine selectivity. It has been demonstrated that observing the amount of nonspecific binding with the SAM will facilitate determination of the SAM’s sufficiency for detecting ADDLs. Higher ratios of TDT:HSA proved to have less nonspecific binding while still maximizing the binding of amyloid β-derived diffusible ligands (ADDLs). It was shown that ADDLs specifically bound to the antibody, producing a red shift in λmax of 12.17 nm. Further work with TDT:HSA may involve advancing this SAM in order to detect ADDLs in living patients.