Solvent Effects on the Kinetics of Amyloid-b OligomerizationPublic Deposited
Amyloid-β oligomers, also known as amyloid-β derived diffusible ligands (ADDLs), have been implicated in numerous studies as being a possible cause for Alzheimer’s disease (AD). The goal of this study was to investigate the kinetics of the oligomerization of the monomer amyloid-β (Aβ) into ADDLs in various ratios of dimethyl sulfoxide (DMSO) and phosphatebuffered saline (PBS). The results indicate that by changing the intermolecular structure of water, DMSO affects protein interactions and possibly hydrogen bonding, both of which are needed in the formation of ADDLs. In addition to affecting water, DMSO appears to affect the ability of Aβ monomers to form hydrogen bonds, which are integral to Aβ oligomerization. This information will eventually be used to study the formation of ADDLs with a localized surface plasmon resonance (LSPR) nanosensor, with the goal of preventing or reversing the formation of ADDLs, thus leading to a cure or treatment for AD.