Proper spatiotemporal expression of genes is essential during development. One method of regulation of signaling-responsive genes is at the level of transcription. In this work, I present the adaptation of single molecule fluorescent in situ hybridization for use in Drosophila imaginal disc tissues in order to more precisely quantify transcript...
Herpesvirus virions consist of three layers: nucleocapsid, tegument, and envelope. The innermost layer, the nucleocapsid, initially assembles as an immature procapsid precursor built around viral scaffold proteins. The event that initiates procapsid maturation is unknown but it is dependent upon activation of the internal protease. Scaffold cleavage triggers angularization, or...
It is becoming increasingly evident that the nanoscale organization and structure of macromolecules play a significant role in determining the function and properties of biological systems. To understand the relationships between biological structure and function at nanometer length scales, there is a need for methods which enable imaging of intact...