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The Design and Application of Novel Biochemical Assays Based on the SAMDI-MS Platform

Description:

SAMDI-MS (Self-Assembled Monolayers for MALDI-TOF Mass Spectrometry) couples the use of chemically-defined self-assembled monolayers of alkane thiolates on gold surfaces with MALDI-TOF mass spectrometry for rapid characterization of the surfaces. Reactions performed on the surfaces can be quantified directly by detection of the reaction substrates and products. This rapid detection...

Keyword:

SAMDI, mass spectrometry, bioassay, chromodomains, cell-free reactions, and microfluidics

Subject:

Analytical chemistry, Materials Science, and Biochemistry

Creator:

O'Kane, Patrick Thomas

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

02/12/2020

Date Created:

2019-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:14615 and etdadmin_upload_661860
High-Throughput Development of Glycosyltransferase Assays Using SAMDI-MS

Description:

More than half of proteins in humans are modified with carbohydrates in a process called glycosylation, yet this process remains poorly understood even though approximately 1% of the expressed human genome encodes biosynthetic machinery for glycosylation. Unlike genomics and proteomics where high throughput tools are now routinely used to generate...

Keyword:

SAMDI, High-throughput protein synthesis, glycosylation, self-assembled monolayers, glycosyltransferase, and Mass spectrometry

Subject:

Bioengineering, Analytical chemistry, and Biochemistry

Creator:

Techner, Jose-Marc

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

02/12/2020

Date Created:

2019-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:14598 and etdadmin_upload_661107

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The Design and Application of Novel Biochemical Assays Based on the SAMDI-MS Platform

High-Throughput Development of Glycosyltransferase Assays Using SAMDI-MS

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