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Filtering by: Creator Adam D. Silverman Language English

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Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry

Description:

Recent advances in cell-free gene expression (CFE) systems have enabled their use for a host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and biosensors. Despite the proliferation of cell-free protein synthesis platforms, the large number of currently existing protocols for making CFE extracts muddles the collective...

Keyword:

cell-free synthetic biology, TX-TL, CFE, cell extract, genetic circuitry, in vitro protein synthesis, CFPS, and endogenous transcription

Subject:

Synthetic biology and Genetic regulation

Creator:

Nancy Kelley-Loughnane, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman

Owner:

Julius Beau Lucks

Publisher:

American Chemical Society

Language:

English

Date Uploaded:

12/14/2018

Date Modified:

01/07/2020

Date Created:

12/14/18

Resource Type:

Dataset
Source Data for "Point-of-use detection of environmental fluoride via a cell-free riboswitch-based biosensor."

Description:

Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that...

Keyword:

Diagnostic, Fluoride, Riboswitch, Cell-Free, and Synthetic Biology

Subject:

Synthetic Biology and Diagnostics

Creator:

Nancy Kelley-Loughnane, Matthew S. Verosloff, Michael C. Jewett, Walter Thavarajah, Julius B. Lucks, and Adam D. Silverman

Contributor:

This work was supported by the Air Force Research Laboratory Center of Excellence for Advanced Bioprogrammable Nanomaterials (C-ABN) Grant FA8650-15-2-5518 (to M.C.J. and J.B.L), the David and Lucile Packard Foundation (to M.C.J.), an NSF CAREER Award (1452441 to J.B.L.), and the Camille Dreyfus Teacher-Scholar Program (to M.C.J. and J.B.L.). A.D.S. was supported in part by the National Institutes of Health Training Grant (T32GM008449) through Northwestern University’s Biotechnology Training Program.

Owner:

Julius Beau Lucks

Language:

English

Date Uploaded:

06/18/2019

Date Modified:

01/08/2020

Date Created:

2019-06-04

Resource Type:

Dataset

Alternate Identifier:

https://doi.org/10.21985/N2RJ64