Cells are often precisely organized into patterns within developing tissues. This precision must emerge from biochemical processes within, and between cells, that are inherently stochastic. I investigated the impact of stochastic gene expression on self-organized pattern formation, focusing on Senseless (Sens), a key target of Wnt and Notch signaling during...
Proper spatiotemporal expression of genes is essential during development. One method of regulation of signaling-responsive genes is at the level of transcription. In this work, I present the adaptation of single molecule fluorescent in situ hybridization for use in Drosophila imaginal disc tissues in order to more precisely quantify transcript...