Proper spatiotemporal expression of genes is essential during development. One method of regulation of signaling-responsive genes is at the level of transcription. In this work, I present the adaptation of single molecule fluorescent in situ hybridization for use in Drosophila imaginal disc tissues in order to more precisely quantify transcript...
The role of nuclei nanoenvironment in cellular function has been a challenging problem in biology due to the lack of chromatin 3-dimension (3D) structure imaging/capturing techniques and theory connecting physical structure of chromatin to transcription. Recent studies on optical properties measurement on biological sample techniques, nanoscale imaging techniques and chromatin...