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Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry

Description:

Recent advances in cell-free gene expression (CFE) systems have enabled their use for a host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and biosensors. Despite the proliferation of cell-free protein synthesis platforms, the large number of currently existing protocols for making CFE extracts muddles the collective...

Keyword:

cell-free synthetic biology, TX-TL, CFE, cell extract, genetic circuitry, in vitro protein synthesis, CFPS, and endogenous transcription

Subject:

Synthetic biology and Genetic regulation

Creator:

Nancy Kelley-Loughnane, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman

Owner:

Julius Beau Lucks

Publisher:

American Chemical Society

Language:

English

Date Uploaded:

12/14/2018

Date Modified:

01/07/2020

Date Created:

12/14/18

Resource Type:

Dataset