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Cell-free biosynthesis of isoprenoids using Escherichia coli crude lysates

Description:

Metabolic engineering of microorganisms to produce useful compounds from renewable substrates is a promising means for sustainable, on-demand production of chemicals. However, efforts to design and engineer microbial cell factories are constrained by costly and slow “build” times in which each genetic variation requires re-engineering a new strain for each...

Keyword:

metabolic engineering, cell-free, synthetic biology, prototyping, and limonene

Subject:

Chemical and Biological Engineering

Creator:

Quentin Merle Dudley

Owner:

Scholarly Digital Publishing

Date Uploaded:

01/25/2019

Date Modified:

01/30/2019

Date Created:

2017-01-01

Resource Type:

Dissertation
A Cell-Free Framework for Rapid Enzymatic Pathway Prototyping and Discovery

Description:

It is pertinent now more than ever that we find sustainable alternatives to produce chemicals. For decades, scientists and engineers have turned to biological systems to help meet societal needs in energy, medicine, materials, and moreespecially when chemical synthesis is untenable. Often, biologically-produced small molecules are insufficient for production at...

Keyword:

metabolic engineering, biotechnology, cell-free systems, biosynthetic pathway, synthetic biology, and enzymatic reactions

Subject:

Chemical and Biological Engineering

Creator:

Ashty Karim

Owner:

Scholarly Digital Publishing

Date Uploaded:

10/14/2019

Date Modified:

11/20/2019

Date Created:

2018-01-01

Resource Type:

Dissertation

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Cell-free biosynthesis of isoprenoids using Escherichia coli crude lysates

A Cell-Free Framework for Rapid Enzymatic Pathway Prototyping and Discovery

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