With the advent of a variety of novel nanometer-scale diagnostic techniques such as DNA micro- and nanoarrays, increasing focus has been placed on developing methods to quickly and accurately create these nanometer scale patterns. One promising technique for depositing DNA, proteins, and other liquid molecular "inks" with submicron resolution is...
Magnetic nanostructures (MNS) have become the subject of intense
interest due to their superparamagnetic properties and consequently
their potential uses in medical diagnostics and therapeutics. In this
study, monodisperse magnetite MNSs were synthesized via thermal
decomposition of iron(III) acetylacetonate and transferred to aqueous
solution using 11-aminoundecanoic acid as the surfactant....
Alzheimer’s disease (AD) is one of the most common forms of dementia,
with no current definitive premortem diagnosis. Amyloid beta-peptide
derived diffusible ligands, known as ADDLs, have become a key link in
the AD specificity for memory loss and are a potential biomarker for AD.
In order to develop an...
Neurons are difficult to manipulate experimentally. Most transfection
reagents used for gene silencing are neurotoxic, and the postmitotic
state of neurons prevents the effective use of viral vectors. This study
demonstrated the delivery of antisense DNA against target genes by
gold nanoparticle oligonucleotide complexes (GNOs) in primary cultures
Thermoelectric materials, which convert heat energy to electrical
energy, may become increasingly important in the world’s energy
situation. Through the inclusion of nanoscale features embedded within
a thermoelectric bulk material, it has been shown that a corresponding
decrease in lattice thermal conductivity results in an enhanced
thermoelectric figure of merit,...
Under the correct conditions, aluminum can be anodized in an electrochemical
cell to produce hexagonally ordered arrays of nanoscale
pores. This study examines how adding the chemicals methanol and
acetic acid to a typical anodization solution alters pore-growth patterns
across the surface oxide layer. The existence of the unique pore-growth...
This paper investigates the use of self-assembled monolayers
(SAMs) to optimize the localized surface plasmon resonance (LSPR)
biosensor for disease detection. Various experiments were done
with 1-(9-mercaptononyl)-3,6,9-trioxaundecan-11-ol (TDT) and
heptaoxatricosanoic acid (HSA), a new SAM; these included a solvent
study to determine refractive index sensitivity and nonspecific binding
Cadmium selenide (CdSe) quantum dots have low conductivity upon
synthesis with the original capping ligands. The conductivity can be
altered by the addition of other ligands. Thiols, amines, and carboxylic
acids, as well as other functional groups, have been shown to improve
conductivity in quantum dot films. This investigation describes...
This study examined the invasiveness of nanofountain probes (NFP)
when used as a transfection tool for single-cell research. For comparison,
the damage caused to cells by more commonly used commercial
atomic force microscope (AFM) probes was also studied. Forces were
applied to cells at increasing levels using both probes, and...
The swelling and deswelling behavior of poly(methacrylic acid) (PMAA)
thin films and the permeability of poly(styrene) (PS) membranes was
investigated using quartz crystal microbalance and ellipsometry.
The system consisted of either homopolymer PMAA film or diblock
poly(methyl methacrylate)-poly(methacrylic acid) (PMMA-PMAA)
brushes with a hydrophobic PS membrane capping layer. Results show...
This paper reports the syntheses of acetylene-terminated smallmolecule
cores. Also reported are successful attempts at “click”
coupling the core molecules with azide-terminated oligonucleotides to
construct stable small-molecule DNA hybrids (SMDH). Once these
click-based small-molecule DNA hybrids are formed, melting studies
will be done that study how the geometry of SMDH...
"This paper describes the development of an efficient transfection
vehicle for nucleic acids using surface-functionalized gold nanoparticles
(AuNPs) with a mixed-monolayer of biological molecules—a hybrid
approach that combines two previously investigated models. The
objective is to improve upon previously described nanoparticles used to
introduce nucleic acids to cells—oligonucleotide-functionalized AuNPs
Semiconductor nanowires show promise for application in nanoscale
electronics, but the difficulty of forming low-resistance ohmic contacts
provides a challenge to their implementation. To improve the electrical
performance of lithographically defined nickel contacts, nickel-silicide/
silicon axial nanowire heterostructures were formed by controlled
partial silicidation. Prior to annealing, two-terminal silicon nanowire...
Small, soluble, self-assembling peptide Aβ-oligomers, known as ADDLs
(amyloid-derived diffusible ligands), accumulate in the Alzheimer’s
disease (AD) brain as Aβ 12-mers and are thought to contribute to AD
neuropathological conditions. Unlike plaques, which were once thought
to cause neuronal death due to insoluble amyloid plaque deposits,
ADDLs initiate abnormal changes...
In the field of clinical diagnostics, biosensors are used to identify genetic
disorders by hybridizing a surface-bound DNA sequence with the
patient’s DNA. To further improve biosensor design, two nonlinear
optical techniques, sum frequency generation (SFG) and second
harmonic generation (SHG), were used to probe the orientation,
chirality, and surface...
The formation of organic layers on hydrogen-passivated silicon (HPS)
surfaces via (UV) ultraviolet treatment was completed for three styrene
derivatives with different conjugated lengths. The styrene molecules
were synthesized and characterized using nuclear magnetic resonance
(NMR) spectroscopy and gas chromatography-mass spectrometry
(GC-MS). Layer formation on silicon was then accomplished through...
Polymer nanoparticles (PNPs) were formed from amphiphilic diblock
copolymers consisting of a hydrophilic block with pendant polyethylene
glycol tosylate (PEG-OTs) groups and a hydrophobic block with pendant
terthiophene (TTT) groups. The resulting PNPs were characterized
using dynamic light scattering (DLS) and transmission electron
microscopy (TEM) to determine size, shape, and...
The formation of covalently grafted polymer brushes on silica (SiO2)
nanoparticles was achieved by surface-initiated reversible additionfragmentation
chain transfer (si-RAFT) polymerization. RAFT initiator
was first attached onto the surface of silica nanoparticles and then
examined by ultraviolet-visible spectroscopy (UV-vis) analysis. From the
resulting RAFT initiator-modified SiO2 nanoparticles, polymer chains
Bone-related problems have always been a concern in the medical field,
particularly how to heal and rebuild bones more rapidly when injuries
occur. A path to solving such problems might be to examine the
processes when bone develops in nature. This project aims at discovering
the chemical reactions that occur...