Little is known about the transcriptional events underlying megakaryocytic (Mk) differentiation from hematopoietic stem cells. We sought to identify genes and pathways not previously associated with megakaryopoiesis and to evaluate the CHRF-288-11 (CHRF) megakaryoblastic cell line as a model system for investigating megakaryopoiesis.
Using DNA microarrays, Q-RT-PCR, and protein-level assays,...
In yeast, inducible genes such as INO1, PRM1 and HIS4 reposition from the nucleoplasm to nuclear periphery upon activation. This leads to a physical interaction with nuclear pore complex (NPC), interchromosomal clustering, and stronger transcription. Repositioning to the nuclear periphery is controlled by cis-acting transcription factor (TF) binding sites located...
This dissertation consists of three chapters on empirical industrial organization. The first chapter explores how a seller uses a public reserve price to signal her private info about the object’s value to the bidder. The second chapter studies how a behavioral consumer preference called “price reference effect” could overturn the...
The phase-field crystal (PFC) model is an exciting new method for simulating crystalline materials with atomic resolution over diffusional time scales. Unfortunately, applications of the model have been severely limited by the requirement that novel free energies must be constructed for each new material of interest. This thesis describes three...
Pseudomonas aeruginosa is a Gram-negative pathogen that frequently causes severe nosocomial infections through expression of virulence factors, evasion of immune clearance and resistance to therapeutic antimicrobial agents. These factors have led the US Centers for Disease Control and Prevention (CDC) and Infectious Disease Society of America (IDSA) to identify P....
The policies of secular governments in constitutionally secular countries are at times marked by the influence of religious politics. Religious politics is specifically defined here as a politics of extending the norms of religion into the public sphere through religious legislation. Thus, some governments lend the state’s coercive capacity to...
The BCR-ABL negative Myeloproliferative Neoplasms (MPNs) are clonal hematopoietic stem-cell disorders characterized by abnormal proliferation of differentiated myeloid lineages. MPNs include 3 clinically distinct disorders: Polycythemia Vera (PV), Essential Thrombocythemia (ET) and Primary Myelofibrosis (PMF). 95% of MPNs are characterized by driver mutations in Janus Kinase 2 (JAK2), Thrombopoietin receptor...
Every music theorist has their own personal reasons for engaging in the craft of theorizing about music. Many discover that there is something special about a theoretic disposition which differs from other sorts of interactions with music. It is often this special something that draws musicians into the discipline: specifically,...
Every music theorist has their own personal reasons for engaging in the craft of theorizing about music. Many discover that there is something special about a theoretic disposition which differs from other sorts of interactions with music. It is often this special something that draws musicians into the discipline: specifically,...
Recently, much public attention has been focused on racial inequalities in who is subjected to exclusionary school disciplinary policies, and consequently, forced to miss hours of instruction. Over the past two decades, researchers have documented the disparate impacts that zero-tolerance policies have had on students of color, low-income students, and...