Synthesis of Fluorescent DNA-Modified Polymer Nanoparticles for Use in a Highly Sensitive DNA Assay,Public Deposited
Polymer nanoparticles (PNPs) were formed from amphiphilic diblock copolymers consisting of a hydrophilic block with pendant polyethylene glycol tosylate (PEG-OTs) groups and a hydrophobic block with pendant terthiophene (TTT) groups. The resulting PNPs were characterized using dynamic light scattering (DLS) and transmission electron microscopy (TEM) to determine size, shape, and polydispersity. Following characterization, PNPs were functionalized with oligonucleotides, and the success of functionalization was verified by hybridization of the DNAmodified PNPs with complementary DNA-functionalized gold nanoparticles (GNPs). DNA-functionalized PNPs were then able to be used as probes in a three-strand DNA detection assay employing the fluorescent and electrochemical properties of the TTT group as a multifunctional signaling unit.