Source Data for "Point-of-use detection of environmental fluoride via a cell-free riboswitch-based biosensor."Public Deposited
Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term storage and detect fluoride at levels above 2 parts per million, the EPA’s most stringent regulatory standard, in both laboratory and field conditions. Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment.
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- This work was supported by the Air Force Research Laboratory Center of Excellence for Advanced Bioprogrammable Nanomaterials (C-ABN) Grant FA8650-15-2-5518 (to M.C.J. and J.B.L), the David and Lucile Packard Foundation (to M.C.J.), an NSF CAREER Award (1452441 to J.B.L.), and the Camille Dreyfus Teacher-Scholar Program (to M.C.J. and J.B.L.). A.D.S. was supported in part by the National Institutes of Health Training Grant (T32GM008449) through Northwestern University’s Biotechnology Training Program.
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