Dopaminergic neurons in the substantia nigra pars compacta are richly innervated by GABAergic neurons. The postsynaptic effects of GABA on SNc DA neurons are mediated by a mixture of GABAA and GABAB receptors. Although activation of GABAA receptors inhibits spike generation, the consequences of GABAB receptor activation are less well characterized. To help fill this gap, perforated patch recordings were made from young adult mouse SNc DA neurons. Sustained stimulation of GABAB receptors hyperpolarized SNc DA neurons, as previously described. However, transient stimulation of GABAB receptors by optical uncaging of GABA did not; rather, it reduced the opening of small-conductance, calcium-activated K+ channels and increased the irregularity of spiking. This modulation was attributable to inhibition of adenylyl cyclase and protein kinase A. Thus, because suppression of SK channel activity increases the probability of burst spiking, transient co-activation of GABAA and GABAB receptors could promote a pause-burst pattern of spiking

Last modified

• 03/22/2018

Creator

• Chad Michael Estep

DOI

• https://doi.org/10.21985/N2X97N

Subject

• Interdepartmental Neuroscience Program (NUIN)

Keyword

• gabab
• pka
• substantia nigra
• dopamine
• sk channel

Date created

• 2016-01-01

Resource type

• Dissertation

Rights statement

• In Copyright