DNA Surface Characterization Using Second Harmonic Generation and Contact-Angle GoniometryPublic Deposited
Chip-based devices and biosensors incorporate the attachment of biomolecules at interfaces. The behavior of biomolecules at the surface and interfaces differs from the behavior of biomolecules in the bulk solution. Surface-analysis techniques are therefore required to characterize these interfaces. Second harmonic generation (SHG) has been used to characterize DNA on a quartz lens to model biomolecules at the surface. Optimization of the surface-attachment procedure using contact-angle goniometry is necessary to optimize the results of laser experiments. Contact-angle goniometry is used to optimize surface preparation parameters, such as concentration of NHS, concentration of water, reaction time, and various cleaning strategies. Studying DNA surfaces can assist with improvements in DNA detection systems for biodiagnostics by determining important structural information about the biomolecules.