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Description:: Recent advances in cell-free gene expression (CFE) systems have enabled their use for a host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and biosensors. Despite the proliferation of cell-free protein synthesis platforms, the large number of currently existing protocols for making CFE extracts muddles the collective...
Keyword:: cell-free synthetic biology, TX-TL, CFE, cell extract, genetic circuitry, in vitro protein synthesis, CFPS, and endogenous transcription
Subject:: Synthetic biology and Genetic regulation
Creator:: Nancy Kelley-Loughnane, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman
Owner:: Julius Beau Lucks
Publisher:: American Chemical Society
Language:: English
Date Uploaded:: 12/14/2018
Date Modified:: 01/07/2020
Date Created:: 12/14/18
Resource Type:: Dataset

Description:: Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that...
Keyword:: Diagnostic, Fluoride, Riboswitch, Cell-Free, and Synthetic Biology
Subject:: Synthetic Biology and Diagnostics
Creator:: Nancy Kelley-Loughnane, Matthew S. Verosloff, Michael C. Jewett, Walter Thavarajah, Julius B. Lucks, and Adam D. Silverman
Contributor:: This work was supported by the Air Force Research Laboratory Center of Excellence for Advanced Bioprogrammable Nanomaterials (C-ABN) Grant FA8650-15-2-5518 (to M.C.J. and J.B.L), the David and Lucile Packard Foundation (to M.C.J.), an NSF CAREER Award (1452441 to J.B.L.), and the Camille Dreyfus Teacher-Scholar Program (to M.C.J. and J.B.L.). A.D.S. was supported in part by the National Institutes of Health Training Grant (T32GM008449) through Northwestern University’s Biotechnology Training Program.
Owner:: Julius Beau Lucks
Language:: English
Date Uploaded:: 06/18/2019
Date Modified:: 01/08/2020
Date Created:: 2019-06-04
Resource Type:: Dataset
Alternate Identifier:: https://doi.org/10.21985/N2RJ64

Description:: Recent advances in cell-free synthetic biology have spurred the development of in vitro molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However, cell-free sensors for detecting manmade organic water contaminants such as pesticides are sparse, partially because few characterized natural biological sensors can directly detect such pollutants. Here,...
Keyword:: biosensor, TX-TL, atrazine, cell-free, cyanuric acid, metabolism, synthetic biology, and transcription factor
Creator:: Umut Akova, Khalid K. Alam, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman
Owner:: Adam Silverman
Date Uploaded:: 09/23/2019
Date Modified:: 02/19/2020

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